Fig. 8.

Knockdown of GADD45α aggravated APAP-induced liver injury in vivo. a The mRNA expression levels of the GADD45α after GADD45α-siRNA transfection in mice. b Liver histological examination with H&E; typical images were chosen from each experimental group. c Serum was harvested at 24 h after APAP injection for the measurement of ALT, AST and LDH in the mice of GADD45α-siRNA and control groups. d Representative images of TUNEL staining after the knockdown of GADD45α in mice at 24 h after APAP treatment and the quantification of the number of TUNEL-positive cells. e Oil Red O staining after the knockdown of GADD45α in mice at 24 h after APAP treatment. f Serum levels of CK18 M65, in the GADD45α-siRNA or control groups. g The mRNA levels of genes involved in fatty acid beta-oxidation, tricarboxylic acid cycle and glycogenolysis in the mice with knockdown of GADD45α after APAP treatment for 24 h. The relative mRNA levels were normalized to 36B4 and subsequently normalized to the control group. Data are expressed as the mean ± standard error of the mean, *P < 0.05, **P < 0.01