Fig. 8.

BH4-Bcl-2 reduces the binding of FLL to LBD. a Typical FRET-based measurements to assess the effect of the BH4-Bcl-2 or the scrambled peptide (BH4-Bcl-2-CTR; scr) on the interaction between FLL and a fluorescent IP₃-binding protein (CFP-coupled LBD). The decrease in binding is shown by an increase in the emission ratio of CFP/FLL (480 nm/535 nm). IP₃ (10 μM) was added at the end of the experiment to obtain the maximal increase in CFP/FLL (maximal decrease of binding). b Quantification of the increase in the FRET signal due to binding of 3, 10 or 30 μM BH4-Bcl-2 expressed as percentage of the maximal increase obtained after addition of 10 μM IP₃. The data from three or four independent experiments are plotted as mean ± SEM. *Stands for p < 0.05