Fig. 3.
The effects of H2O2, cumene hydroperoxide (CMHP) and various plant natural products on the redox status of the Prx1m protein in T. thermophila. The T. thermophila cells were exposed to the indicated concentrations of either the peroxides or the plant natural products for a period of 10 min and then the total protein was extracted from the cells and subjected to NR-PAGE. Equal amounts of protein were loaded into each of the lanes of the gel. Following the NR-PAGE step, the proteins were transferred to nitrocellulose and the Prx1m protein was detected using anti-(T. thermophila Prx1m) antibodies. The monomer and dimers forms of the Prx1m protein are located at ~ 28 and ~ 56 kD, respectively. The molecular size markers were supplied by Bio-Rad