Table 2.
Predicted subcellular locations and molecular weights (MW) of the Prx1 proteins from a selection of ciliates and apicomplexans
| Species | Protein | Accession number | Score | Location | MW (kD) | ||
|---|---|---|---|---|---|---|---|
| mTP | SP | Other | |||||
| Ciliates | |||||||
| T. thermophila | Prx1a | XP_001029987.1 | 0.10 | 0.32 | 0.60 | – | 23 |
| Prx1b | XP_001031522.1 | 0.42 | 0.05 | 0.52 | – | 26 | |
| Prx1m | EAR92904.2 | 0.73 | 0.24 | 0.10 | M | 28 | |
| Prx1c | EAR84222.2 | 0.50 | 0.31 | 0.30 | – | 21 | |
| S. lemnae | Prx1a | CDW79681.1 | 0.11 | 0.92 | 0.03 | S | 23 |
| Prx1b | CDW72826.1 | 0.11 | 0.08 | 0.90 | – | 29 | |
| Prx1m | CDW79455.1 | 0.90 | 0.05 | 0.10 | M | 25 | |
| I. multifiliis | Prxla | EGR27018.1 | 0.30 | 0.11 | 0.61 | – | 24 |
| Prxlb | EGR27550.1 | 0.20 | 0.11 | 0.70 | – | 23 | |
| Prxlc | EGR30706.1 | 0.01 | 1.00 | 0.14 | S | 24 | |
| Prxld | EGR31994.1 | 0.10 | 0.24 | 0.70 | – | 23 | |
| Prxle | EGR29838.1 | 0.20 | 0.93 | 0.10 | S | 25 | |
| Prx1m | EGR29584.1 | 0.60 | 0.05 | 0.42 | M | 22 | |
| O. trifallax | Prx1a | EJY85734.1 | 0.20 | 0.91 | 0.02 | S | 23 |
| Prx1b | EJY73729.1 | 0.34 | 0.10 | 0.44 | – | 28 | |
| Prx1c | EJY85449.1 | 0.10 | 0.05 | 0.94 | – | 51 | |
| Prx1d | EJY82209.1 | 0.10 | 0.05 | 0.94 | – | 46 | |
| Prx1e | EJY81461.1 | 0.34 | 0.10 | 0.61 | – | 24 | |
| Apicomplexans | |||||||
| P. falciparum | Prx1a | XP_001348542.1 | 0.14 | 0.10 | 0.82 | – | 22 |
| Prx1m | XP_001350554.1 | 0.90 | 0.04 | 0.14 | M | 22 | |
| T. gondii | Prx1a | EPR63775.1 | 0.11 | 0.20 | 0.80 | – | 22 |
| Prx1m | EPR64157.1 | 0.93 | 0.03 | 0.11 | M | 22 | |
| C. parvum | Prx1a | ACV31867.1 | 0.23 | 0.11 | 0.60 | – | 22 |
| E. tenella | Prx1a | XP_013230312.1 | 0.20 | 0.10 | 0.80 | – | 21 |
| Prx1m | CDJ42921 | 0.97 | 0.04 | 0.03 | M | 28 | |
| T. annulata | Prx1a | CAI76104.1 | 0.11 | 0.12 | 0.80 | – | 22 |
| Prx1b | CAI73861.1 | 0.40 | 0.06 | 0.52 | – | 29 | |
| B. microti | Prx1a | XP_012647735.1 | 0.10 | 0.20 | 0.80 | – | 22 |
| Prx1m | XP_012647852.1 | 0.94 | 0.02 | 0.10 | M | 22 | |
The proteins were named according to the new naming system recently proposed by Gretes et al. [1]. According to this system, when there are multiple Prx1 proteins, they are designated ‘a’, ‘b’, ‘c’, etc., except for mitochondrial Prx1 proteins which are designated ‘m’. The subcellular locations were predicted using TargetP version 1.1 (http://www.cbs.dtu.dk/services/TargetP/). The MW values were predicted using the “Compute pI/MW” program accessed via the ExPASy Bioinformatics Resource Portal (https://www.expasy.org/resources). The MW values exclude the mitochondrial targeting peptide when one is present. The abbreviations mTP, SP, M and S indicate mitochondrial targeting peptide, secretory pathway signal peptide, a mitochondrial protein and a secreted protein, respectively. The scores indicate the likelihood of the prediction being correct with 1.0 being the highest possible value