Fig. 2.

Analysis of apoptosis induction by miR-493-3p. The ovarian CA cell lines SKOV3, OVCAR3, TOV21G, TOV112D, A2780 as well as A2780-cis were seeded 24 h prior transfection with 62.5 nM miR-493-3p mimics, non-targeting siRNA (NT, negative control for cell death), cell death inducing siRNA (DT, positive control for cell death) or treatment with 25 µM Etoposide, 80 µM Carboplatin or 0.25 µM Paclitaxel. 48 h after treatment, the cells were analysed for their release of cytochrome C (b) as well as the loss of ∆Ψm (c). 72 h after treatment the cell confluency (a) and the fragmentation of DNA (d) was analysed. 5 µM CCCP served as a positive control for the breakdown of ∆Ψm (c). Statistical analysis was performed by one-way ANOVA followed by Bonferroni post-test. [n = 3 replicates; mean ± SD, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001]