Fig. 7.

STK38L is phosphorylating RAF1. To demonstrate an interaction of STK38L with RAF1, immunoprecipitations were carried out. Protein lysates of SKOV3 cells were precipitated for RAF1 or STK38L and blotted against both proteins (a). To obtain more information about the interaction between STK38L and RAF1, HEK293T cells were transfected with overexpression plasmids of Flag tagged STK38L Wild Type (Flag-STK38L WT) or a kinase dead version of STK38L (Flag-STK38L KD). The protein lysates were precipitated for RAF1 and blotted for total RAF1 and phosphorylated RAF1 (Ser621) (b, left part). The lysates were blotted against the Flag tag to demonstrate equal STK38L overexpression (b, right part). GAPDH served as loading control. [n = 2 replicates] The regulation of RAF1 by STK38L and ETS1 is illustrated schematically in part d. Furthermore, the signalling pathways leading to apoptosis in ovarian CA cell lines are shown schematically in part c