Fig. 2.

EGF-induced cell proliferation is dependent on uPAR activity. a EGF promotes cell proliferation. HT-29 cells were treated with increasing doses of EGF ranging from 1 ng/ml (0.16 nM) to 100 ng/ml (16 nM). Cell number was estimated after 4 days of culture and is represented as mean ± SD from three experiments. Number of cells kept in serum-free media without EGF is referred to as 100% proliferation (control). *p < 0.05 and **p < 0.01 compared to control. b EGF-promoted cell proliferation is blocked by anti-uPAR antibodies. The effects of EGF (5 ng/ml = 0.80 nM) on HT-29 cell proliferation were determined in the presence or in the absence of either SI517 or SI518 (each at 40 μg/ml) anti-uPAR polyclonal antibody. An unrelated antibody (40 μg/ml) serves as negative control. Data are the mean ± SD (n = 3). Statistical significance is p < 0.05 in an ANOVA model, for 10% FCS, EGF and EGF + unrelated Ab conditions