Table 1.
Detected homologues of L. distinctifemur venom proteins
| Species | Pristhesancus plagipennis | Cimex lectularius | Rhodnius prolixus | Panstrongylus herreri | Triatoma lectularia | Halyomorpha halys | Triatoma pallidipennis | Triatoma infestans | Lygus hesperus | Triatoma infestans | Rhodnius robustus and Rhodnius breviceps | Sitobion avenae and Metopolophium dirhodum |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Phylogeny | Heteroptera: Cimicomorpha: Reduviidae: Harpactorinae | Heteroptera: Cimicomorpha: Cimicidae: Cimicinae | Heteroptera: Cimicomorpha: Reduviidae: Triatominae | Heteroptera: Cimicomorpha: Reduviidae: Triatominae | Heteroptera: Cimicomorpha: Reduviidae: Triatominae | Heteroptera: Pentatomomorpha: Pentatomidae: Pentatominae | Heteroptera: Cimicomorpha: Reduviidae: Triatominae | Heteroptera: Cimicomorpha: Reduviidae: Triatominae | Heteroptera: Cimicomorpha: Miridae: Mirinae | Heteroptera: Cimicomorpha: Reduviidae: Triatominae | Heteroptera: Cimicomorpha: Reduviidae: Triatominae | Sternorrhyncha: Aphidoidea: Aphididae: Aphidinae |
| Trophic strategy | Predation (invertebrates) | Haematophagy | Haematophagy | Haematophagy | Haematophagy | Phytophagy | Haematophagy | Haematophagy | Phytophagy (facultative predation) | Haematophagy | Haematophagy | Phytophagy |
| Study | Walker et al. (2017, 2018) | Francischetti et al. (2010) | Montandon et al. (2016) | Montandon et al. (2016) | Montandon et al. (2016) | Peiffer et al. (2014) | Hernandez-Vargas et al. (2017) | Assumpcao et al. (2008) | Cooper et al. (2013) | Charneau et al. (2007) | Costa et al. (2011) | Rao et al. (2013) |
| Methods | Venom gland transcriptomics and MS/MS of venom | Venom gland transcriptomics and MS/MS of venom glands | MS/MS of venom (against previous transcriptomics) | MS/MS of venom (against previous transcriptomics) | MS/MS of venom (against previous transcriptomics) | MS/MS of saliva (against previous transcriptomics) | Venom gland transcriptomics and MS/MS of venom | Venom gland transcriptomics and MS/MS of venom | MS/MS of saliva (against previous transcriptomics) | MS/MS of venom (against previous transcriptomics) | Venom gland transcriptomics and MS/MS of venom | MS/MS of saliva (against previous transcriptomics of different species) |
| No. BLASTp hitsa | 82 | 46 | 39 | 36 | 5 | 37 | 7 | 6 | 6 | 3 | 1 | 1 |
| No. non-S1 protease BLASTp hitsa | 34 | 13 | 3 | 2 | 4 | 2 | 6 | 6 | 6 | 3 | 1 | 1 |
| All BLASTp hitsa (%) | 62.1 | 34.8 | 29.5 | 27.3 | 3.8 | 28 | 5.3 | 4.5 | 4.5 | 2.3 | 0.8 | 0.8 |
| Non-S1 protease BLASTp hitsa (%) | 34.7 | 13.3 | 3.1 | 2 | 4.1 | 2 | 6.1 | 6.1 | 6.1 | 3.1 | 1 | 1 |
Proteomes of venom/saliva secretions of other heteropteran insects determined by transcriptomics and proteomics were BLAST searched using L. distinctifemur venom proteins as queries
aBLAST hits with E < 0.01