Table 2.
BLAST searches of hemipteran genomes for genes encoding venom protein homologues
| Protein family | With giant water bug sequences as query | With assassin bug sequences as query | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Pristhesancus plagipennis | Pachypsylla venusta | Diuraphis noxia | Philaenus spumarius | Diaphorina citri | Pseudococcus longispinus | Lethocerus distinctifemur | Pachypsylla venusta | Diuraphis noxia | Philaenus spumarius | Diaphorina citri | Pseudococcus longispinus | |
| High confidence ID in L. distinctifemur and P. plagipennis venom but not reported elsewhere | ||||||||||||
| CUB domain | 2E−12 | 2.2 | > 10 | > 10 | 1.3 | > 10 | 1E−06 | 0.032 | 0.41 | 1.2 | 0.52 | 0.71 |
| Haemolysin-like | 2E−21 | 0.21 | > 10 | 0.99 | 1.1 | 0.011 | 5E−20 | 0.022 | 3.1 | 1.4 | 0.48 | > 10 |
| Venom family 1 | 0.001 | 9.5 | > 10 | > 10 | > 10 | > 10 | 0.002 | 1.3 | 0.84 | 5.1 | 4.4 | 0.35 |
| Venom family 2 | 1E−10 | 0.16 | 0.21 | 0.1 | 7E−03 | 0.01 | 3E−06 | 0.54 | 0.61 | 1.3 | 0.75 | 1.5 |
| Venom family 5 | 0.039 | 1.3 | > 10 | > 10 | 1.6 | 3.3 | 0.061 | 1.6 | 9.7 | 0.15 | 2.4 | 1.1 |
| Venom family 10 | 1E−06 | 0.5 | 6.9 | > 10 | 0.77 | > 10 | 1E−06 | > 10 | 10 | 3.2 | 6.3 | > 10 |
| Venom family 12 | 3E−13 | > 10 | > 10 | > 10 | > 10 | > 10 | 1E−113 | 4.7 | > 10 | 0.87 | 1.5 | 2.7 |
| High confidence ID in L. distinctifemur and P. plagipennis venom and previously known | ||||||||||||
| CRiSP | 5E−37 | 1E−04 | 0.11 | 6E−06 | 6E−04 | 4E−06 | 1E−28 | 2E−06 | 0.002 | 7E−06 | 6E−04 | 2E−06 |
| Cystatin | 5E−09 | 2E−05 | > 10 | 0.05 | 5E−05 | 0.98 | 1E−09 | 2.2 | 4.1 | 0.82 | 0.29 | 0.5 |
| IPPase | 1E−77 | 8E−29 | 1E−32 | 2E−07 | 5E−09 | 4E−31 | 1E−78 | 1E−28 | 1E−35 | 1E−09 | 1E−08 | 7E−32 |
| Lipase | 3E−64 | 2E−18 | 4E−26 | 3E−13 | 2E−15 | 3E−29 | 5E−63 | 7E−17 | 2E−33 | 3E−21 | 6E−17 | 2E−55 |
| Lysozyme | 5E−24 | 6E−04 | > 10 | 3E−04 | 0.003 | 3.5 | 3E−25 | 2E−09 | > 10 | 3E−12 | 1E−11 | 2.4 |
| Nuclease | 3E−139 | 2E−23 | 2E−11 | 2E−25 | 2E−22 | 1E−52 | 3E−133 | 8E−20 | 4E−12 | 9E−19 | 8E−15 | 3E−49 |
| Pacifastin | 4E−25 | 0.056 | > 10 | 0.17 | 0.12 | > 10 | 2E−23 | 0.009 | > 10 | 0.36 | 0.075 | > 10 |
| POBP | 3E−37 | 8E−15 | 5E−10 | 1E−13 | 1E−14 | 1E−11 | 4E−37 | 3E−13 | 6E−12 | 3E−13 | 2E−13 | 7E−13 |
| S1 protease | 6E−124 | 7E−17 | 2E−22 | 4E−14 | 1E−11 | 4E−19 | 4E−132 | 9E−79 | 3E−60 | 1E−86 | 2E−76 | 1E−91 |
| Serpin | 6E−127 | 2E−24 | 8E−34 | 6E−35 | 1E−20 | 1E−59 | 9E−116 | 2E−05 | 1E−26 | 8E−13 | 2E−07 | 2E−35 |
| Transferrin | 0 | 9E−172 | 1E−22 | 3E−19 | 1E−139 | 3E−07 | 0 | 3E−164 | 2E−23 | 5E−18 | 5E−129 | 9E−07 |
In each case examined, L. distinctifemur and P. plagipennis venom proteins are more similar to each other than any non-heteropteran sequences