. 2019 Mar 19;76(12):2369–2382. doi: 10.1007/s00018-019-03071-y

Table 1.

Specifications of the different stationary phase polymer types used as SEC matrix for EV isolation

SEC matrix	Polymer type	Particle size (μm)^a	Fractionation range (kDa)		Exclusion limit (kDa)^b	Exclusion limit (nm)^d	%^e
SEC matrix	Polymer type	Particle size (μm)^a	Protein M^b_r	Dextrans M^c_p	Exclusion limit (kDa)^b	Exclusion limit (nm)^d	%^e
Sepharose CL-2B	2% cross-linked agarose	60–200	70–40,000	100–20,000	40,000	75	45.8
Sepharose CL-4B	4% cross-linked agarose	45–165	60–20,000	30–5000	20,000	42	10.4
Sepharose CL-6B	6% cross-linked agarose	40–165	10–4000	10–1000	4000	24	2.1
Superose 6	Cross-linked agarose	8.6	5–5000	1–300	40,000	29	6.3
Superdex 200	Cross-linked agarose and dextran	8.6	10–600	1–100	1300	13	16.7
Sephacryl S-200 HR	Cross-linked allyl dextran and N,N′-methylene bisacrylamide	25–75	5–250	1–80	250	7.7	2.1
Sephacryl S-300 HR		25–75	10–1500	2–400	2000	13	0
Sephacryl S-400 HR		25–75	20–8000	10–2000	8000	31	6.3
Sephacryl S-500 HR		25–75	–	40–20,000	20,000	42	6.3
Sephacryl S-1000 SF	Spherical allyl dextran and N,N′-methylene bisacrylamide	40–105	–	500–100,000	100,000	400	4.2

The SEC matrix types enumerated are available from different manufacturers both as bulk chromatography resin or as already-made columns for bench-top use and also compatible with automatic chromatography systems, e.g. FPLC systems

^aDiameter of the matrix particles

^bMolecular weight of globular proteins

^cPeak molecular mass of dextrans

^a,b,cValues according to manufacturers

^dMatrix pore sizes according to [90], unless stated by manufacturer

^ePercentage of works using the corresponding SEC resin out of the total scientific publications using SEC for EV isolation (EV-Track database available in January 2019; n = 48)