Table 1.
List of strains used in this study
| Strain | Genotype | Origin |
|---|---|---|
| Y610 | MATα pph22::HA-PPH21 TRP1::GAL1-FLAG3-ZDS1 CDC14-MYC18 cdc20::MET-CDC20 | This study |
| Y888 | MATa cdc55::HA3-CDC55 TRP1::GAL1-FLAG3-ZDS1 CDC14-Pk9 cdc20::MET-CDC20 | This study |
| Y769 | Matα cdc20::MET-CDC20 TRP1::GAL1-FLAG3-ZDS1 cdc55::HA3-CDC55 | This study |
| Y770 | Matα cdc20::MET-CDC20 cdc55::HA3-CDC55 TRP1::GAL1-FLAG3-zds1Δ803-916 | This study |
| Y1015 | Mata cdc20::MET-CDC20 TRP1::GAL1-FLAG3-ZDS1 cdc55::HA3-CDC55 CDC14-Pk9 NET1-GFP | This study |
| Y2541 | Mata cdc20::GAL1-CDC20 cdc55::HA3-CDC55 | This study |
| Y492 | Mata cdc20::GAL1-CDC20 cdc55::HA3-CDC55 cdc28::cdc28-as1 | This study |
| Y537 | MATa HA3-CDC55 TRP1::GAL1-FLAG3-ZDS1 cdc55::HA3-CDC55 CDC14-Pk9 cdc20::MET-CDC20 cdc28::cdc28-as1 | This study |
| Y1125 | Mata cdc20::MET-CDC20 clb2Δ TRP1::GAL1-FLAG3-ZDS1 cdc55::HA3-CDC55 CDC14-Pk9 | This study |
| Y569 | MATa cdc20::MET-CDC20 cdc55::HA3-CDC55 | This study |
| Y480 | MATa cdc20::MET-CDC20 cdc55::HA3-CDC55 cdc28::cdc28-as1 | This study |
| Y1281 | MATa cdc20::MET-CDC20 cdc55Δ URA3::HA3-CDC55 NET1-MYC18 | This study |
| Y1284 | MATa cdc20::MET-CDC20 cdc55Δ URA3::HA3-CDC55-2A NET1-MYC18 | This study |
| Y1081 | MATa cdc20::MET-CDC20 cdc55Δ URA3::HA3-CDC55 GAL1-FLAG3-ZDS1 | This study |
| Y1087 | MATa cdc20::MET-CDC20 cdc55Δ URA3::HA3-CDC55-2A GAL1-FLAG3-ZDS1 | This study |
| Y1211 | Mata cdc20::MET-CDC20 cdc55Δ URA3::HA3-CDC55 CDC14-Pk9 | This study |
| Y1215 | Mata cdc20::MET-CDC20 cdc55Δ URA3::HA3-CDC55-T174A CDC14-Pk9 | This study |
| Y1216 | Mata cdc20::MET-CDC20 cdc55Δ URA3::HA3-CDC55 -S301A CDC14-Pk9 | This study |
| Y1217 | Mata cdc20::MET-CDC20 cdc55Δ URA3::HA3-CDC55 -2A CDC14-Pk9 | This study |
| Y3034 | MATa cdc20::MET-CDC20 zds1∆ CDC14-Pk9 | Our lab |
| Y564 | MATa cdc20::MET-CDC20 cdc55::HA3-CDC55 | This study |
| Y879 | Mata cdc20::MET-CDC20 cdc55Δ CDC14-Pk9 | Our lab |
| Y1212 | Mata cdc20::MET-CDC20 cdc55Δ URA3::HA3-CDC55-T174E CDC14-Pk9 | This study |
| Y1213 | Mata cdc20::MET-CDC20 cdc55Δ URA3::HA3-CDC55-S301D CDC14-Pk9 | This study |
| Y1214 | Mata cdc20::MET-CDC20 cdc55Δ URA3::HA3-CDC55-ED CDC14-Pk9 | This study |
| Y500 | Mata cdc20::GAL1-CDC20 cdc55::Pk3-CDC55 | This study |
| Y2312 | MATa cdc20::GAL1-CDC20 CDC14-HA6 CLB2-Pk3 | Our lab |
| Y1049 | MATa cdc20::MET-CDC20 cdc55Δ URA3::HA3-CDC55 | This study |
| Y1077 | MATa cdc20::MET-CDC20 cdc55Δ cdc55::HA3-CDC55 | This study |
| Y1080 | MATa cdc20::MET-CDC20 cdc55Δ URA3::HA3-CDC55-2A | This study |
| Y824 | MATα cdc20::MET-CDC20 | Our lab |
| Y1294 | MATa cdc20::MET-CDC20 cdc55Δ URA3::HA3-CDC55-ED NET1-MYC18 | This study |
| Y1285 | MATa cdc20::MET-CDC20 cdc55Δ URA3::HA3-CDC55 ZDS1-PK6 | This study |
| Y639 | MATa cdc20::MET-CDC20 NET1-MYC18 CDC14-Pk9 | Our lab |
| Y1118 | MATa cdc20::MET-CDC20 zds1∆zds2∆ cdc55::HA3-CDC55 YCplac22 | This study |
| Y1192 | MATa cdc20::MET-CDC20 zds1∆zds2∆ cdc55::HA3-CDC55 YCplac22-Zds1Promoter-FLAG3-ZDS1_full length | This study |
| Y1119 | MATa cdc20::MET-CDC20 zds1∆zds2∆ cdc55::HA3-CDC55 YCplac22-Zds1Promoter-FLAG3-zds1∆461-916 | This study |
| Y1122 | MATa cdc20::MET-CDC20 zds1∆zds2∆ cdc55::HA3-CDC55 YCplac22-Zds1Promoter-FLAG3-zds1∆1-802 | This study |
| Y1117 | MATa cdc20::MET-CDC20 zds1∆zds2∆ cdc55::HA3-CDC55 YCplac22-Zds1Promoter-FLAG3-zds1∆74-802 | This study |
| Y1120 | MATa cdc20::MET-CDC20 zds1∆zds2∆ cdc55::HA3-CDC55 YCplac22-Zds1Promoter-FLAG3-zds1∆461-802 | This study |
| Y1288 | MATa cdc20::MET-CDC20 cdc55Δ URA3::HA3-CDC55-ED ZDS1-PK6 | This study |
| Y1291 | MATa cdc20::MET-CDC20 cdc55Δ URA3::HA3-CDC55-2A ZDS1-PK6 | This study |
| Y1597 | MATα cdc20::MET-CDC20 zds1Δ zds2Δ cdc28::cdc28Y19F cdc55Δ CDC14-HA6 pRS316 | This study |
| Y1598 | MATα cdc20::MET-CDC20 zds1Δ zds2Δ cdc28::cdc28Y19F cdc55Δ CDC14-HA6 pRS316-Pk3-CDC55 | This study |
| Y1600 | MATα cdc20::MET-CDC20 zds1Δ zds2Δ cdc28::cdc28Y19F cdc55Δ CDC14-HA6 pRS316-Pk3-CDC55-ED | This study |
| Y536 | MATa cdc55::HA3-CDC55 | This study |
| Y567 | MATa cdc55::HA3-CDC55 esp1::esp1-2 | This study |
| Y568 | MATa cdc55::HA3-CDC55 esp1::esp1-2 | This study |
| Y565 | MATa cdc20::MET-CDC20 cdc55::HA3-CDC55 esp1::esp1-2 | This study |
| Y888 | MATa cdc20::MET-CDC20 TRP1::GAL1-FLAG3-ZDS1 cd55::HA3-CDC55 CDC14-Pk9 | This study |
| Y524 | MATa cdc20::MET-CDC20 esp1::esp1-2 TRP1::GAL1-FLAG3-ZDS1 cdc55::HA3-CDC55 CDC14-Pk9 | This study |
| Y2782 | MATα cdc20::MET-CDC20 esp1::esp1-2 cdc55Δ CDC14-HA6 pRS316 | This study |
| Y1561 | MATα cdc20::MET-CDC20 esp1::esp1-2 cdc55Δ CDC14-HA6 pRS316-Pk3-CDC55 | This study |
| Y1481 | MATα cdc20::MET-CDC20 esp1::esp1-2 cdc55Δ CDC14-HA6 pRS316-Pk3-CDC55-ED | This study |
| Y755 | MATa cdc55::HA3-CDC55 ZDS1-FLAG3 | This study |
| Y986 | MATa cdc20::MET-CDC20 cdc55Δ | Our lab |
| Y616 | Mata cdc20::MET-CDC20 pph22::PPH21-HA | This study |
| Y1649 | MATa cdc20::MET-CDC20 cdc55Δ URA3::HA3-CDC55 | This study |
| Y1651 | MATa cdc20::MET-CDC20 cdc55Δ URA3::HA3-CDC55-2A | This study |
| Y3227 | MATa cdc20::GAL1-CDC20 CDC14-Pk9 | Our lab |
| Y3034 | Mata cdc20::MET-CDC20 zds1Δ CDC14-Pk9 | Our lab |