Fig. 2.

Isolation of microvesicle-like structures. a Scheme of MVs’ isolation protocol. b Laser-scattering trace of isolated MVs. A mean diameter of 380 nm was measured in the MVs’ sample (top panel). In contrast, a mean diameter of 63 nm was measured in exosomes sample (bottom panel). Representative graph shows the size profile of one analyzed sample. c Negative stain TEM analysis of the enriched MVs’ sample shows vesicle within the range size of MVs. d Regular TEM analysis of the enriched MVs’ sample shows the presence of structures surrounded by membrane with different shapes and sizes ranging from 100 nm up to 1 µm