Fig. 5.

VHH 4.80 blocks leptin protection against starvation-induced splenic and thymic atrophy. C57BL/6 mice were fed ad libitum or were starved for 48 h. Starved animals were injected i.p. at the start of the starvation and 24 h later with PBS, pegylated leptin or a combination of pegylated leptin and 4.80-mAlb. Animals were killed, thymi (a, b) and spleens (c, d) isolated and weighed (a, c) and the total number of white blood cells (b, d), splenic lymphocytes (e), neutrophils (f) and monocytes (g) counted using the Hemavet instrument. Values represent mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.005 vs. ad libitum-fed controls, unless stated otherwise. Data representative for three independent experiments