Fig. 6.

SIRT3 protein level is reduced in cellular models of cystinosis and recovered by cysteamine treatment. Proteins of cellular lysate of the different cellular models were loaded on 8% SDS-PAGE, transferred to nitrocellulose membranes, and immunoblotted with the antibody against SIRT3. Protein loading was assessed by reprobing the blots with the actin antibody. The histograms represent the means values ± SEM of the ratio of ADU (arbitrary densitometric units) of the immune-revealed SIRT3 normalized on actin level (Student’s t test)