Fig. 7.

Cysteamine or 8Br-cAMP treatment ameliorates mitochondrial morphology in cystinotic cells and in HK-2 cells silenced for CTNS gene. a Representative images of mitochondrial network of cells stained with MitoTracker^® Orange CMTMRos and a z-stack of optical sections, 10 µm in total thickness, were captured by fluorescence microscopy. Scale bars correspond to 25 µm. b, c Reported the quantification of mitochondrial fragmentation index (f index) in ciPTEC and HK-2 cells treated with 100 µM cysteamine (MEA) or 100 µM 8Br-cAMP for 24 h. Data are the mean values (± SEM) of the analyses performed in cell numbers of n = 20 for ciPTEC cells and n = 30 for HK-2 cells. Significant differences were calculated with Student’s t test