Fig. 1.

Expression of CDH22 in developmental ovaries and in FGSCs. Examination of CDH22 expression (brown) in 5-day (A), 10-day (B), 20-day (C) and 42-day (D) ovaries using IHC. Red arrows indicate FGSCs in the cortex. Bar = 50 µm. The morphology of FGSCs after ten passages maintained on STO feeders (E-a). FGSCs were identified with BrdU/MVH dual immunostaining (E-b). Immunofluorescence was used to detect the expression of CDH22 (E-c), β-catenin (E-d) and Oct4 (E-e) in FGSCs in vitro. The expression of germ line markers in FGSCs was evaluated with RT-PCR; 1, 2, and 3 represent cDNA from the ovary, FGSCs and feeders, respectively (F), and the expression of a series of known stem cell and germ cell markers was examined in FGSCs, combined with BrdU immunofluorescence (G, red), including β-catenin (G-f), E-cadherin (G-g), CDH22 (G-h), Fragilis (G-i), ITGA6 (G-j), ITGB1 (G-k), JAK2 (G-l) and STAT3 (G-m) (green). Bar = 20 µm