Fig. 4.

Iristatin’s effect on leukocyte recruitment and migration and macrophage production of NO. a–f Mice were injected with 3% TGM with saline or Iristatin. After 4 h, peritoneal lavage was performed and infiltrating cells analyzed by flow cytometry. In all figures, mice injected with TGM and saline are marked as Untreated, while all mice injected with TGM and Iristatin (2 mg/kg of mouse weight) are labeled as Iristatin. a Total number of cells in the peritoneum in tested mice. b Percentage of all living cells in mouse peritoneum. c–f Total number of all living CD11b⁺ cells, neutrophils, monocytes, or eosinophils, respectively. g Mouse bone marrow neutrophils were preincubated with 3 μM Iristatin and subjected to migration towards fMLP in a Boyden chamber. h Iristatin inhibited NO production by PMJ2-R macrophages in a dose-dependent manner. Macrophages were preincubated with 3 and 6 μM Iristatin, stimulated with LPS and IFN-γ, and NO concentration was assessed after 24 h. The mean of three independent experiments (± SEM) is shown. *P ≤ 0.05; **P ≤ 0.01; n.s. not significant