Fig. 5.

Influence of NANOS gene mutations on SIAH1 mRNA regulation. a Mutations related to human infertility in NANOS proteins are schematically depicted. NIM and zinc-finger domains are indicated as dark rectangles, and mutations identified in patients manifesting reproductive system failure are presented in bold. b Influence of NANOS NIM region mutations on expression of luciferase reporter carrying SIAH1 3′UTR. c Effects of mutated NANOS proteins (from patients diagnosed with failure of the reproductive system) on expression of luciferase reporter carrying SIAH1 3′UTR (P < 0.05, P < 0.005, P < 0.001). Error bars denote standard deviation (n = 9). Luminescence values (RLU relative luciferase units) are presented as % relative to the sample transfected with the reporter construct only which was set to 100%