Fig. 1.

TSHR in interaction with MCT8. The analysis was performed via YFP-based bimolecular fluorescence complementation assay (YFP-BiFc) in transiently transfected HEK293 cells. The expression of TSHR-YFP1 and the co-expression of TSHR/control (rM3R) (white bars) served as negative control, homodimers of TSHR and MCT8, respectively, served as positive controls. The bars represent results from the pooled co-expression values from vice versa assays (f.e. TSHR+MCT8: TSHR-YFP1/MCT8-YFP2 and TSHR-YFP2/MCT8-YFP1). Data were assessed from a minimum of three independent experiments (50.000 cells counted per experiment) and calculated to 100% TSHR dimer. Values represent mean + SEM. Significances are shown compared to the co-expression of TSHR/control. *p ≤ 0.05, ***p ≤ 0.001 (unpaired t test, two-tailed)