Fig. 5.
Gs-signaling of TSHR co-expressed with MCT8. Gs-activity (cAMP) was measured with the AlphaScreen technology in transiently transfected COS-7 cells after stimulation with bTSH (range 0.01–100 mU/mL bTSH). The co-expression of TSHR with the non-interactive rM3R (control) was used to ensure equal DNA amount. Cells transfected with empty vector showed no response in cAMP accumulation to bTSH (data not shown). Values represent raw data mean + SEM from at least three independent experiments, performed in triplicates. Statistical analysis was performed using two-way ANOVA which did not reveal any statistical differences between the concentration response curves of TSHR + control and TSHR + MCT8