Table 1.
Original FCCS fitting parameters (± S.E.) for the GFP- and mCherry-tagged constructs in the plasma membrane of transiently transfected HEK293 cells
| Constructs | τD,1 (%) | τD,1 (ms) | τD,2 (%) | τD,2 (ms) | N | G(0)x/G(0)min |
|---|---|---|---|---|---|---|
| MCT8-GFP | 36.8 ± 6.7 | 0.45 ± 0.02 | 63.2 ± 0.8 | 44.9 ± 2.2 | 69 | 20.8 ± 0.3 |
| TSH-mCherry | 50.0 ± 2.5 | 0.26 ± 0.04 | 50.0 ± 2.4 | 26.4 ± 7.3 | ||
| CRF1-GFP | 38.5 ± 0.8 | 0.45 ± 0.03 | 61.5 ± 0.8 | 40.8 ± 2.5 | 51 | 17.8 ± 0.2 |
| CRF1-mCherry | 60.0 ± 1.8 | 0.24 ± 0.04 | 40.0 ± 1.8 | 20.2 ± 2.1 | ||
| CRF2a-GFP | 40.6 ± 1.1 | 0.37 ± 0.02 | 59.4 ± 1.1 | 36.5 ± 1.4 | 63 | 9.4 ± 0.3 |
| CRF2a-mCherry | 60.5 ± 1.4 | 0.19 ± 0.04 | 39.5 ± 1.4 | 22.7 ± 1.9 |
Decay times [τD,1 (ms); τD,2 (ms)], fractions [(τD,1 (%); τD,2 (%)], and CC values [G(0)x/G(0)min] are listed. The best fits were obtained assuming free diffusion in two dimensions and two components. Triplet fractions [TF (%)] ranged from 8.34–40.83% and triplet times (τD,F) from 5.00 to 159.97 µs