Fig. 4.

HBeAg-induced miR-155 expression in different kinds of macrophages. RAW264.7 macrophages were stimulated with HBeAg for 24 or 36 h, and microRNA sequencing assay was performed according to the manufacturer’s protocols (a). After HepG2 or HepG2.2.15 and RAW264.7 cells were co-cultured with transwell for 24, 36, or 48 h, the expression of miR-155 and BIC was measured with q-PCR (b). RAW264.7 macrophages were stimulated with HBeAg for 24, 36, or 48 h, and miR-155 and BIC expression was evaluated with q-PCR (c). Mouse primary peritoneal macrophages (d) and peripheral blood monocytes of health controls (e) were treated with HBeAg for 36 h, and miR-155 and BIC expression was evaluated with q-PCR. Data are representative of three independent experiments (mean ± SD of triplicates in b, c and quadruplicates in d, e). *p < 0.05, **p < 0.01, ***p < 0.001