Fig. 3.

AFAP1-AS1 promotes tumor cell migration and invasion in lung cancer cells. a Images of cells with or without AFAP1-AS1 overexpression under fluorescence microscope. b Semi-quantitative RT-PCR analysis of AFAP1-AS1 expression in H1299 cells with or without AFAP1-AS1 overexpression. Actin mRNA was used as control. c Cell migration and invasion assays of H1299-control or H1299-AFAP1-AS1 cells. d Schematic illustration of locations of qRT-PCR primers (primer 1/2) and two independent shRNAs (shAS1 and shAS2) in AFAP1-AS1 gene. Semi-quantitative RT-PCR (e) and qRT-PCR analyses (f) of AFAP1-AS1 expression in the stable transfectants of PC9 and H1975 cells. g Remarkably reduced cell migration and invasion in PC9 cells (left) and H1975 cells (right) following knockdown of AFAP1-AS1 by shRNAs