Fig. 4.

JNK and c-Src inhibitors SP600125 and PP2 increased the pHe in cells with impaired CFTR activity. a pHe measured in conditioned media from Caco-2/pRSctrl cells (open bars) and Caco-2/pRS26 (closed bars) cells, previously incubated in serum-free medium for 24 h, and then treated for 48 h with inhibitors of the IL-1β pathway (5 μM IKK-2 inhibitor SC-514, 10 μM c-Src inhibitor PP2, 5 μM P38 inhibitor SB203580, 5 μM MEK1/2 inhibitor U0126, and 5 μM JNK inhibitor SP600125) with the replenishment of fresh media at time zero. *p < 0.05 compared to Caco-2/pRSctrl cells. One-way ANOVA and Tukey´s test for n = 3 independent experiments were performed. b Dose–response curve corresponding to pHe measured in conditioned media from Caco-2/pRSctrl cells (solid line) and Caco-2/pRS26 (dotted line) cells, treated for 48 h with the JNK inhibitor SP600125. c Dose–response obtained by incubation with the c-Src inhibitor PP2 in the same conditions as b. The dose–response curves were fitted with a sigmoidal function and t values were calculated from R² (p < 0.001 in b and c). *p < 0.05, comparing the values of Caco-2/pRS26 at each concentration with the values of Caco-2/pRSctrl cells at the same concentration, using the Student t test (n = 4 independent experiments). Kruskal–Wallis test and the Mann–Whitney test were used to corroborate significant difference by a non-parametric analysis and the results were equivalent