Fig. 6.

Oxamate treatment normalized the pHe and lactate values of Caco-2/pRS26 cells. a pHe was measured in conditioned media from Caco-2/pRSctrl and Caco-2/pRS26 cells, previously incubated in serum- free medium for 24 h, and treated with different oxamate concentrations (0–60 mM) for 48 h. pHe vs oxamate concentration points were fitted using a sigmoidal (dose–response) function fixing the low asymptote to 0 mM concentration and the t values were calculated from the R² (p < 0.001). b Lactate secreted into the culture media of Caco-2/pRSctrl and Caco-2/pRS26 cells treated with 60 mM oxamate, at 0, 5, 24, and 48 h). ***p < 0.001 with respect to pRS26 cells at 48 h (ANOVA one-way-Tukey´s test). c Intracellular LDH activity of Caco-2/pRSctrl and Caco-2/pRS26 cells treated with 60 mM oxamate for 48 h. LDH activity was expressed as % and referred to Caco-2/pRSctrl as 100% of activity. *p < 0.05 with respect to Caco-2/pRS26 cells at 48 h (ANOVA one-way-Tukey´s test, n = 3 independent experiments). Kruskal–Wallis test and the Mann–Whitney test were used to corroborate significant difference by a non-parametric analysis and the results were equivalent