Table 3.

Cumulative inactivation of Kv currents in adipocytes

Group of cells	Insulin (µIU/ml)	Percentage of cells with cumulative inactivation	Percentage of cumulative inactivation
<10% inactivation	10	50	11.7 ± 0.4 (2)
	100	54.5	16.1 ± 3.1 (6)
10–30% inactivation	10	60	11.5 ± 2.9 (6)
	100	70	14.2 ± 1.9 (14)
>30% inactivation	10	41.2	15.4 ± 5.2 (7)
	100	46.7	13.9 ± 3.5 (7)

Adipocytes were differentiated in poor (< 10%), moderate (10–30%) and pronounced (> 30%) inactivation of Kv currents. SVF-derived adipocytes were incubated 4–7 days in low (10 µIU/ml) or high (100 µIU/ml) insulin concentrations following what described in the methods section. The cumulative inactivation was measured applying a train of seven depolarizing voltage steps of 250 ms from − 60 to + 60 mV once every 400 ms. The percentage of inactivation was calculated from the remaining current at the end of the last pulse vs the first. Values are mean ± SE. Number of cells indicated in brackets