Fig. 4.

Cdk6 is downstream of p27 in hippocampal progenitors. a Cdk6 or p27 immunoprecipitation was carried out on protein lysates from 4- to 6-week-old mice hippocampi and followed by western blot using p27 or Cdk6 antibody, respectively. Corresponding western blots were performed on crude cell extracts (inputs). b Histogram showing the number of Ki67⁺ cells per mm² of DG in WT (n = 5), p27 KO (n = 6), Cdk6 KO (n = 4), Cdk6 KO/p27 KO (n = 5), R31C (n = 3) and R31C/p27 KO (n = 3) mice. Data are presented as mean ± SEM and were analyzed by one-way ANOVA followed by a Newman–Keuls post hoc test. **p < 0.01; ***p < 0.001. c Western blot analysis of cyclin D2 expression in protein extracts from P6 to P7 WT, p27 KO, R31C and R31C/p27 KO hippocampi. Each well corresponds to one independent animal. β-actin serves as a loading control. ns not significant