Fig. 2.

a h20S (114 nM) was incubated in the presence and absence of 200 nM IDE for 10 min at 37 °C. The samples were separated under native conditions and probed with the LLVY-amc fluorogenic substrate (left panel). The identity of the particles was further assessed by WB (right panel). b h20S (57 nM) was allowed to interact with h19S (250 nM) in the presence and in the absence of 200 nM IDE. The complexes were resolved by native gel electrophoresis and the particles probed with 100 µM LLVY-amc. The identity of the complexes was further probed by WB. A representative immunoblot of five independent experiments is shown