Table 2.

Interaction parameters according to Scheme 1 between IDE and h20, the wt y20S proteasome and its mutant α3ΔN

	Human	Yeast	α-3ΔN
1KIDE (M)	1.3 (± 0.3) × 10−8	1.6 (± 0.3) × 10−8	1.6 (± 0.4) × 10−8a
2KIDE (M)	8.9 (± 1.3) × 10−8	7.4 (± 0.9) × 10−8	7.4 (± 0.9) × 10−8a
Chymotrypsin-like
α	1.5 ± 0.3	2.2 ± 0.4	1.0a
β	0.79 ± 0.09	0.35 ± 0.05	1.0a
γ	0.46 ± 0.07	0.9 ± 0.2	0.64 ± 0.08
δ	1.2 ± 0.3	3.0 ± 0.5	1.0 ± 0.2
Trypsin-like
α	1.5 ± 0.3
β	0.72 ± 0.09
γ	0.46 ± 0.07
δ	1.15 ± 0.20
Caspase-like
α	1.5 ± 0.3
β	0.01 ± 0.01
γ	0.46 ± 0.07
δ	0.66 ± 0.08

^aThese values are kept fixed