Table 3.
Semi-cloned embryo developmental potential
| Species | Gender of ES cell lines | Experimental procedure | Embryos transferred at the 2-cell stage | Full-term pups (% of reconstructed embryos) | Survive to adulthood (% of full-term pups) | Germline transmission ability | References |
|---|---|---|---|---|---|---|---|
| Medaka fish | PG-haESCs | Bimaternal | 667a | 7 (1) | 1 (14) | Yes | [29] |
| Mouse | PG-haESCs | ICPI | 290 | 2 (0.7) | 1 (50) | Yes | [21] |
| Mouse | AG-haESCs | ICAI | 424b | 9 (2.1) | 8 (89) | Yes | [14] |
| Mouse | AG-haESCs | ICAI | 913c | 29 (3) | 12 (41) | Yes | [13] |
| Mouse | DKO AG-haESCs | ICAI | 1993 | 402 (20.2) | NDd | Yes | [40] |
| Mouse | DKO AG-haESCs | ICAI | 400 | 41 (10) | 21 (51) | ND | [36] |
| Mouse | DKO AG-haESCsDnmt3b | ICAI | 305 | 31 (10) | 28 (90.3) | ND | [36] |
| Mouse | DKO PG-haESCs | Bimaternal | 1019 | 158 (15.5) | NDd | Yes | [22] |
| Mouse | PG-haESCsΔIG/Δ5H19/Igf2 | Bimaternal | 450 | 32 (7.1) | 23 (71.9) | Yes | [44] |
| Mouse | PG-haESCsΔIG/Δ5H19 | Bimaternal | 475 | 43 (9) | 31 (72) | Yes | [44] |
| Mouse | TKO PG-haESCs | Bimaternal | 210 | 29 (14) | 27 (93) | Yes | [65] |
| Mouse | 7KO AG-haESCs | Bipaternale | 477 | 12 (2.5) | 0 (0) | / | [65] |
| Rat | AG-haESCs | ICAI | 1142 | 2 (0.2) | 2 (100) | Yes | [23] |
| Rat | Transgenic AG-haESCs | ICAI | 441 | 4 (0.9) | 1 (25) | ND | [23] |
ND not determined
aNumber of reconstructed embryos
bNumber of blastocysts
cNumber of 2-cell embryos and blastocysts from AG-haESCs and gene-modified AG-haESCs
dMost semi-cloned mice grew to adulthood, but specific data are not available
eThese bipaternal mice were produced via a tetraploid embryo complementation assay