Fig. 1.

Rab6 silencing promotes cell migration. a U2OS cells transfected with control siRNA or 4 different siRNAs against Rab6 (#1, #2, #3, #4) were scratch-wounded and imaged every 3rd hour for 24 h. Representative images of (T₀) and 18 h after scratching are shown. Scale bar: 300 µm. b Quantification of the relative wound density (%) as function of time for control cells and Rab6-depleted cells. Data represents the mean of three independent experiments. ***P < 0.001 compared to control (two-way repeated measures ANOVA followed by Tukey’s post test for t = 24 h). c Cell lysates from each sample were subjected to Western blot analysis with antibodies against Rab6 and tubulin (as a loading control). d Representative track plots of the single-cell distances of migration for cells transfected with siRNA control, siRNA Rab6#1 or siRNA Rab6#2. Individual tracks are shown so that each starts at the origin (distance 0). Quantification of the mean ± SEM of speed (e) and directness (f) relative to the control. n > 150 cells from at least five independent experiments. *P < 0.05; **P < 0.01 (paired Student’s t test). g Lysates from cells transfected with either siRNA control or siRNA against Rab6 (#1 and #2) were subjected to Western blot analysis with the indicated antibodies