Fig. 1.

Fibroblast adhesion and migration depends on LRP1. a Wild-type and LRP1^−/− mouse embryonic fibroblasts (MEF) adhesion on plates coated with collagen (COL), fibrinogen (FG), fibronectin (FN), vitronectin (VN) or bovine serum albumin (BSA) as assessed by crystal violet staining. b Adhesion of primary human lung fibroblasts (pHLF) transfected with scrambled (scr) or LRP1-targeting siRNA to FN measured by crystal violet staining. c Time course of wild-type and LRP1^−/− MEF adhesion on FN as assessed by crystal violet assay. d Representative bright-field image stills of live-cell imaging of wild-type and LRP1^−/− MEF during adhesion to FN. Scale bar: 50 µm (n = 3). e Migration of wild-type and LRP1^−/− MEF as assessed by gap closure assay. f Phosphorylation of focal adhesion proteins in wild-type and LRP1^−/− MEF during adhesion on FN investigated by western blotting. g Western blot for Paxillin and FAK phosphorylation in scr or anti-LRP1 siRNA-transfected pHLF at 15 min after seeding onto FN-coated plates. Data in a–c, e are mean ± SEM of three to five experiments; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. Western blots in f and g are representative of three experiments