Fig. 7.

Loss of LRP4 results in activation of Notch signaling. a Dual luciferase reporter assay to quantify the Notch signaling activity as Hes1 was coexpressed with the indicated Lrp4 cDNA in HEK293T cells. bLRP4 siRNA in hFOB cells achieved 69% knockdown with upregulated gene expressions of NOTCH signaling components. c Gene expressions of Notch signaling components were downregulated when Lrp4 was overexpressed stably in MC3T3-E1 cells. Values in a–c represent mean ± SE of data from three independent experiments, *P < 0.05, **P < 0.01. (Student’s t test)