Table 1.
Proteins with cholesterol (PDB code: CLR) bound to their structure
| PDB ID | Protein/lipid/detergent used during purification/crystallization | Bounda | CRACb motifs | CARC motifs | Binding CRAC/CARCc |
|---|---|---|---|---|---|
| X-ray diffraction | |||||
| 1LRI | Fungal elicitor cryptogein | 1 | None | None | No |
| 5XRA | CB1/ monoolein | 1 | None | None | No |
| 2RH1 | β-2 adrenergic receptor/ monoolein | 3 | None | None | No |
| Na+/K+ ATPase/PC | |||||
| 2ZXE | α subunit | 1 | 5 | 12 | No |
| β subunit | 3 | 7 | Yes | ||
| 3WGU | α subunit | 6 | 4 | 14 | No |
| β subunit | 5 | 9 | Yes | ||
| 4XT1 | Human chemokine CX3CL1/1-oleoyl-R-glycerol | 2 | 3 | 3 | No |
| μ-opioid receptor/monoolein | |||||
| 4DKL | 1 | None | None | No | |
| 5C1M | 1 | 3 | 2 | No | |
| 4XP9 | Dopamine transporter/DDM, PE | 1 | 4 | 11 | No |
| 1N83 | Nuclear receptor ROR-alpha | 1 | 1 | 2 | Likely |
| 5LWE | Chemokine receptor type 9/DDM | 1 | 3 | 6 | Yes |
| 5I6X | Human serotonin transporter/PC, PE, PG | 1 | 6 | 5 | No |
| 5L7D | Human smoothened protein/DDM | 1 | None | None | No |
| 3N9Y | Human CYP11A1 | 2 | 7 | 6 | No |
| 5JQH | β-2 adrenoceptor/ monoacylglycerol | 2 | None | None | No |
| 4XNV | P2Y purinoceptor 1/DDM | 1 | None | None | No |
| 5IU4 | A2A adenosine receptor | 4 | None | None | No |
| 5X93 | Endothelin B receptor/ LMNG | 1 | None | None | No |
| 5WVR | OSH1 OSBP-related domain | 1 | 8 | 8 | Yes |
| 1ZHY | Oxysterol binding protein Osh4 | 1 | 3 | 11 | Yes |
| 5TCX | Human tetraspanin CD8/monoolein | 1 | 1 | 5 | No |
| 3GKI | N-terminal domain of Niemann-Pick C1 protein | 1 | None | 4 | No |
| 4BOE | Tick lipocalin japanin | 1 | 4 | 4 | Yes |
| 4OR2 | Metabotropic glutamate receptor 1/monoolein | 6 | None | None | No |
| 4IB4 | Chimera of 5-HT2B-BRIL/monoolein | 1 | None | None | No |
| 3AM6 | Proton pumping rhodopsin AR2/monoolein | 8 | 2 | 3 | No |
| 4PXZ | P2Y12 receptor/monoolein | 1 | None | None | No |
| Electron microscopy | |||||
| 5SY1 | STRA6 receptor/ lauryl maltose neopentyl glycol | 2 | 3 | 10 | No |
| 3JD8 | Niemann-Pick C1 protein | 1 | 6 | 18 | No |
| Small angle neutron scattering | |||||
| 3K2S | High density lipoprotein/POPC | 20 | 5 | 4 | No |
Included proteins have less than 90% sequence similarity (date of analysis: 29th September 2017). Cholesterol-like molecules have not been included into the search
PC 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, PE 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine, PG 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol, DDM n-dodecyl-β-d-maltopyranoside, LMNG lauryl maltose neopentyl glycol
aTotal number of cholesterol molecules bound per structure
bCRAC motifs per chain
cCholesterol motif present within 7 Å radius of one of the three defining residues of a CRAC or CARC motif