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Sequence of the peptide and it analog with observed and calculated mass with HPLC retention time
| Peptide | Sequence | Observed mass | Calculated mass | Retention time (min) |
|---|---|---|---|---|
| MD54 | LKGSKGLLHIFYIPRR | 1898.50 | 1897.2 | ~ 13–14 |
| MMD54 | LKGSKGLAHIFYIPRR | 1856.42 | 1855.14 | ~ 12–13 |
In MMD54, the alanine residue, placed in place of the leucine residue, has been marked in bold
