Fig. 3.

Porf-2 inhibits axon outgrowth via its GAP domain. a The construct scheme of the domain-deleted plasmids of Porf-2. b Verification of domain-deleted plasmids via immunoblotting with the HA antibody. The same blot was probed with β-actin antibody as a loading control. c Representative images of neurons in different groups. Scale bar: 25 μm. d, e Quantification of the neurite length and number in each group. All experiments were performed ≥ 3 times independently. Error bars represent ± SEM; **P < 0.01, ***P < 0.001