Fig. 5.

EphB1 is identified as a Porf-2 binding protein. a The proteins of NG108 cells transfected with Flag-Porf-2 or vector were immobilized on beads via Flag antibody. The immobilized NG108 proteins were used to pull down the proteins from the hippocampal lysate. The proteins pull-down were subject to sliver staining and further identified by mass spectrometry analysis. The red arrow indicates the band of EphB1 protein which was pulled down by anti-Flag antibody. b Screening the interaction of Porf-2 with EphA and EphB family members in NG108 cells. c Pull-down assay to verify the interaction of Porf-2 with endogenous EphB1 in the brain. The proteins of NG108 cells transfected with Flag-Porf-2 or vector were used to pull down the hippocampal proteins, which were further detected by indicated antibodies