Fig. 6.

Porf-2 responds to ephrin-B-EphB signal transduction. a CO-IP assay to explore the binding ability of Porf-2 with EphB1 in response to ephrin-B2-Fc treatment in NG108 cells transfected with Flag-Porf-2 and EphB1-HA. b Endogenous Porf-2 formed clusters (arrowheads) and co-localized with EphB1 clusters (arrowheads) after ephrin-B2-Fc stimulation in primary cultured neuron. The white arrowheads indicate the clustered Porf-2 and EphB1. Scale bar: 5 μm. The co-localized cluster puncta was quantified in each group. c Co-IP assay shows the binding of Porf-2 with EphB1 but not EphB1-ΔC in NG108 cells. d Transfected Porf-2 formed clusters and co-localized with EphB1 clusters but not EphB1-ΔC clusters after ephrin-B2-Fc stimulation in NG108 cells. Scale bar: 10 μm. The red arrowhead in the upper panel indicates no Porf-2 clusters and EphB1 clusters after Con-Fc treatment. The whiter arrows in the middle panel indicate the co-localization of Porf-2 clusters and EphB1 clusters after ephrin-B2-Fc treatment. The white arrowheads in the lower panel indicate the EphB1-ΔC clusters, but no Porf-2 clusters after ephrin-B2-Fc treatment