Fig. 8.

Porf-2 is required for EphB1/Rac1 signal transduction upon ephrin-B2 treatment. a Representative images of neuronal morphology in the shCtrl, shPorf-2 and ΔGAP-Porf-2 groups upon ephrin-B2-Fc treatment. Scale bar: 25 μm. b Quantification of the neurite length and number in each group. c Representative images of phalloidin staining in growth cone of shCtrl-, shPorf-2- and ΔGAP-Porf-2-transfected neurons upon ephrin-B2-Fc treatment. Scale bar: 7.5 μm. d Quantification of the F-actin staining area and number of protrusions in different groups. e The levels of F-actin and Rac1-GTP were detected via immunoblotting in shCtrl, shPorf-2 and ΔGAP-Porf-2 groups upon ephrin-B2-Fc treatment. f The band intensity of immunoblotting in e was quantified and normalized to the shCtrl group. All experiments were performed ≥ 3 times independently. Error bars represent ± SEM; **P < 0.01, ***P < 0.001