Fig. 3.

Correlation of WNT11 with hypertrophic/osteogenic markers and suppression of BMP- and IHH targets by WNT inhibition. A: Micromass pellets of MSCs were treated with 2 µM IWP-2 or the respective amount of DMSO as control starting at day 14 of chondrogenic culture in the presence of TGF-β. Pearson correlation of WNT11 with expression of ALPL, IBSP, and MEF2C after chondrogenesis (n = 20 from 5 independent populations). b qPCR assessment of specified genes at day 21 and day 35. Data are presented as mean ± SEM of n = 5 independent populations