Fig. 4.

Generation of DMD KO and PLN^R14del pigs via CRISPR/Cpf1-mediated gene editing and SCNT. a Photograph of newborn DMD KO cloned piglets. b All DMD KO cloned piglets were genotyped by Sanger sequencing. c Photograph of newborn PLN^R14del cloned piglets. d Identification of newborn PLN^R14del piglets by PCR-Sal I digestion. The two small DNA fragments digested by Sal I restriction enzyme indicate the ssODN-mediated point mutation occurred at the target site. e All PLN^R14del piglets were genotyped by Sanger sequencing