Fig. 3. Perchlorate-controlled ribozyme nuclease activity.

a Gel images of hammerhead self-cleavage assay performed in the presence of rN₆ and different chaotropic solutions. Reaction conditions consisted of 200 mM Tris-HCl pH 8, 100 nM Hammerhead strand A and B, 250 µM rN₆, 2.5 mM magnesium. b, c graphical representation of a exhibiting perchlorate denaturing catalytically unproductive hammerhead-rN₆ complexes, allowing hammerhead renaturation and cleavage. Error bars represent the standard error of the mean with n = 3 independent experiments. Figure 3b, c was created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.