Table 2.

Antibody sources and dilutions

Antigen	Pretreatment	Dilution	Code Number	Clone	Source
NapsinA (M)	High pH 30 min — 96 °C	1/500	NCL-L-Napsin A	IP64	Leica Biosystems
TTF-1 (M)	High pH 30 min — 96 °C	1/2000	M3575	8G7G3	Dako, Agilent
p40 (M)	High pH 60 min — 96 °C	1/400	API 3079 G3	BC28	Biocare Medical
Chromogranin-A (M)	High pH 60 min — 98 °C	1/100	M0869	Dak-A3	Dako, Agilent
Synaptophisin (M)	High pH 15 min — 96 °C	1/200	M7315	Dak-Synap	Dako, Agilent
Smarca4 (M)	High pH 40 min — 96 °C	1/100	sc-17796	G-7	Santa Cruz
PDL1 (M)	High pH 15 min — 96 °C	1/50	SK006	22c3	Dako, Agilent
Pou2f3 (P)	Low pH 15 min — 96 °C	1/200	HPA019652	Polyclonal	Sigma-Aldrich
Aim2 (P)	Low pH 15 min — 96 °C	1/200	HPA031365	Polyclonal	Sigma-Aldrich
Foxi1 (P)	High pH 30 min — 96 °C	1/500	HPA071469	Polyclonal	Sigma-Aldrich

M, monoclonal; P, Polyclonal; Ki-67, Ki67 index; TTF-1, thyroid transcription factor 1; Pou2f3, POU class 2 homeobox 3; Gli1, glioma-associated oncogene family zinc finger 1; Yap1, Yes1 associated transcriptional regulator; Aim2, absent in melanoma 2; Foxi1, forkhead box I1