Fig. 4.

The alleviated mitochondrial oxidative stress and improved mitochondrial biogenesis in vitro and in vivo after CI/R injury by SIRT1. A The level of MDA, B the activity of SOD and (C) GSH-Px; n = 3. D DHE representative fluorescence images and (E) quantification of fluorescence intensity in neurons; n = 3. F qPCR analysis of the PGC-1α, G NRF-1, and (H) TFAM levels; n = 4. I Immunoblot analysis and (J) quantification of the PGC-1α, K NRF-1 and (L) TFAM levels; n = 4. M qPCR analysis of the relative mtDNA amount; n = 4