Figure 4.

TAZ-KO cardiac muscle shows enrichment of nuclear FOXO1. Total mRNA and whole-cell lysate were extracted from both WT and TAZ-KO mouse cardiac tissues, and FOXO1 mRNA (A) and protein (B) were measured using real-time qPCR and WB analysis, respectively. A total protein stain was used for WB analysis normalization (TPN). Data are presented as fold-change relative to WT, with each lane representing an individual biological replicate. (C) Real-time qPCR was used to measure the mRNAs of four FOXO1 substrates (Cdkn11, Gabarapl1, Gadd45γ, and Plk2) using Actb as the internal control. Data points represent mean ± S.D. (error bars) for each individual biological replicate of each group. * 0.05 < p < 0.1, ** 0.01 < p < 0.05, *** 0.001 < p < 0.01.