Figure 5.
Breakdown of transmitter use across the D. melanogaster nervous system
(A) Our neuron-level transmitter predictions across the female optic lobes and central brain and a male ventral nerve cord (see STAR Methods).
(B) Bar plots for the numbers of neurons predicted for different transmitter usages in each super class in the FAFB-FlyWire dataset.50
(C) Schematic of a neuron broken into its neuronal compartments. Inset, the proportion of presynapses in each of the four compartment types.
(D) Synaptic budget across different connection types in FAFB-FlyWire (left) and HemiBrain (right). Heatmaps show the proportion of synaptic contacts from neurons of different predicted transmitter types (columns) used in different inter-compartmental connection types (rows). FAFB-FlyWire, 9,123; hemibrain, 10,122 neurons.
(E) Scaled density plots showing neuronal polarity by neuron-level transmitter prediction. Upper, distribution of projection scores, which is the distance in Euclidean space between the dendritic an axonic midpoint. Lower, segregation index: the higher the score, the more polarized the neuron.73
(F) Scaled density plots showing the distribution of excitation-inhibition balance (proportion of excitatory, acetylcholine, input minus the proportion of inhibitory input; GABA, glutamate) across neuron-level transmitter predictions and compartments. Vertical dashed line, median value. Colored boxes with stars indicate statistical comparisons, Wilcoxon two-sample tests (n.s., not significant; ∗p ≤ 0.05; ∗∗∗p ≤ 0.0001; ∗∗∗∗p ≤ 0.00001).
See also Figures S2 and S3 and Data S3 and S4.