Figure S5.
The distribution of neuron-level transmitter predictions within secondary hemilineages, related to Figure 7
(A) Consistency of neuron-level transmitter predictions within selected hemilineages in the central adult D. melanogaster brain. Bar plots show the proportion of neurons in each hemilineage predicted to express each of our six transmitters. Data is shown for neurons of the left (left) and right (middle) hemispheres of the FAFB-FlyWire dataset, as well as both hemispheres of the HemiBrain dataset (right). Note that the HemiBrain dataset is only a partial brain, many brain neurons have large missing portions or do not exist in this dataset. Hemilineage names are given on the left of the bar plots, and the numbers of neurons per hemilineage are on the right. The red bar highlights lineages of cholinergic Kenyon cells, MBp1-4, which are mispredicted dopaminergic. The plot is faceted first by presence in the HemiBrain dataset (intact, truncated, missing), then by lineage type (Type I and Type II).
(B) Empirical cumulative density plot shows how consistent a transmitter within each hemilineage is predicted to be. The Y axis gives the proportion of hemilineages, and the X axis gives the proportion of neurons in those hemilineages that “voted" for the top transmitter (color groups).
(C) How the Shannon entropy (base 6) in the neuron-level transmitter predictions for each hemilineage correlate, between the hemilineage copy on the right (X axis) and left (Y-axis) hemispheres of the FAFB-FlyWire dataset.
(D) Dot plot shows the mean normalized pairwise NBLAST scores47 between neurons expressing the majority transmitter within a hemilineage (for each green dot, each member of pair expresses the main transmitter) and between these neurons and those expressing other transmitters (pink, at least 10 neurons expresses the other transmitter). Dots represent means taken per hemilineage (183).
(E) Violin plot shows the distribution of neuron-level transmitter prediction confidences for neurons that are in agreement with their hemilineage’s transmitter use (green, strictly obey Lacin’s law) and those that do not (pink, strictly obey Lacin’s law).
(F) Majority unilateral, left-side antennal lobe local neurons of ‘ALlv1 dorsal’. Neurons colored by their neuron-level transmitter prediction except for a minority of predicted serotonergic neurons with the most ventral cel bodies, given in purple. These are the most similar to described Krasavietz positive cholinergic local neurons.63,189 The upper plot shows neurons predicted to transmit acetylcholine with neurons likely mispredicted to transmit serotonin; they have similar primary neurite and soma positions. We suspect they all should be predicted for acetylcholine. Lower, GABAergic predicted neurons have been added in.
(G) Majority bilateral, left-side antennal lobe local neurons of ‘ALlv2’. Neuron meshes colored by neuron-level transmitter prediction. Data were compared using Wilcoxon two-sample tests. Significance values: ns: p > 0.05; ∗: p ≤0.05; ∗∗: p ≤ 0.01; ∗∗∗: p ≤0.001; ∗∗∗∗: p ≤ 0.0001.