Fig. 2.

Optimized workflows used to assess inflammatory and cell signaling proteins in H292 cells exposed to AqEs from different tobacco product categories. A) Cells aimed for inflammatory protein analysis were exposed for 2 hrs to AqE from the test products, followed by recovery in complete culture medium for 48 hrs. At the end of the recovery period, the culture supernatant was kept for multiplex quantitative analysis. B) Cells aimed for phosphoprotein analysis were exposed for 2 hrs to AqE from the test products and the cell lysates were immediately harvested for multiplex semi-quantitative analysis. In both cases, cells were assessed for viability by CellTiter-Glo.