Table 2.
Phosphoproteins included in multiplex assay panel A (phospho-plex).
| Protein Name | Uniprot Entry | Phospho Residue | Description | Function |
|---|---|---|---|---|
| AKT (pan) |
P31749 P31751 Q9Y243 |
S473 | Rac-alpha/beta/gamma serine-threonine protein kinase | Metabolism, proliferation, cell survival, cell growth, angiogenesis |
| GSK-3α/β |
P49840 P49841 |
S21/9 | Glygogen synthase kinase alpha & beta | Glugose homeostasis, Wnt signalling, regulation of transcription factors, apoptosis |
| MEK1 | Q02750 | S218/222 | Dual specificity mitogen-activated protein kinase kinase 1 | Cell growth, survival, differentiation |
| ERK1/2 |
P28482 P27361 |
T202/Y204 | Mitogen-activated protein kinase-1 & 3 | Cell growth, adhesion, survival, differentiation |
| AKT1S1 (PRAS40) | Q96B36 | T246 | Proline-rich AKT1 substrate 1 | Cell growth, survival |
| c-JUN | P05412 | S63 | Transcription factor AP-1 | Cell cycle progression, proliferation, angiogenesis, apoptosis, response to stress |
| SMAD3 | P84022 | S423/425 | Mothers against decapentaplegic homolog 3 | Wound healing, cell differentiation, apoptosis, inflammation |
| NFkB | P19838 | S536 | Nuclear factor NF-kappa-B p105 subunit | inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis |
| p38 MAPK | P53778 | T180/Y182 | Mitogen-activated protein kinase 12 | DNA-damage response, response to stress, cell cycle arrest |
| RS6 | P62753 | S235/236 | 40 S ribosomal protein S6 | Cell growth, proliferation, apoptosis |
| STAT3 | P40763 | Y705 | Signal transducer and activator of transcription 3 | Cell growth, proliferation, migration, differentiation, apoptosis, inflammation, defense response |
| IRS1 | P35568 | S636/639 | Insulin receptor substrate 1 | Glucose homeostasis, PI3K signaling |
| FAK1 | Q05397 | Y397 | Focal adhesion kinase-1 | Cell migration, adhesion, cell cycle, progression, proliferation, apoptosis |
| CREB1 | P16220 | S133 | Cyclic AMP-responsive element-binding protein 1 | Proliferation, migration, tumour suppression, response to nicotine |
| HSP27 | P04792 | S78/82 | Heat shock protein beta-1 | Oxidative stress response, apoptosis, angiogenesis, chemotaxis |
| NRF2 | Q60795 | S40 | Nuclear factor erythroid 2-related factor 2 | Oxidative stress response, angiogenesis |
| PTN11 | Q06124 | Y542 | Tyrosine-protein phosphatase non-receptor type 11 | MAPK signaling, cell adhesion, DNA damage response |