Plasma samples and platelet activation collected downstream of the metal wires during ex vivo whole blood testing. (A) Plasma levels of TAT were measured from downstream blood sampling from the metal wires. Values significantly increased over time (p < 0.0001), but no change was observed between metal types (p = 0.311). (B) PF4 released into the blood downstream of the metal wires also showed a significant increase in PF4 released from the thrombus over time (p < 0.0001), but with no changes between any of the different metal wire groups (p = 0.938). (C) Quantification of plasma levels of sGPVI from downstream of the metal wires also showed a significant increase over time (p < 0.0001), but with no changes between any of the different metal wire groups (p = 0.985). On the platelet surface, the mean fluorescent intensities of (D) P-selectin from downstream blood without CRP-XL stimulation, (E) P-selectin with 0.1 μg/mL CRP-XL, and (F) P-selectin with 1 μg/mL CPR-XL also changed over time at all concentrations (p = 0.008, p < 0.0001, p = 0.011, respectively) with an increase seen over time for 0 μg/mL CRP-XL and decreases seen over time for 0.1 μg/mL CRP-XL and 1 μg/mL CRP-XL. No statistical differences were seen between the metal types for P-selectin (p = 0.165, p = 0.845, p = 0.085, respectively).